Microbial Examination of Milk Sample from Nagpur Region with Reference to Coliform

The study was undertaken to investigate the microbiological quality of raw milk samples and pasteurized samples were used to study from surrounding area of Nagpur and Wardha district, 10 raw sample and 10 pasteurized samples used. Bacteriological identification reveals a definite dominance of E. coli and E. aerogene sp. beside its, the other genera Salmonella typhi were isolated on selective agar medium. The microbial colonies were found to be high in six sample and colony content low in rest four sample. In pasteurized sample the coliform colonies were less in number as compared to raw milk sample. Bacteriological colonies were found to be opaque and metallic sheen in coloured prepared. Biochemical test were used for the of identified Escherichia coli and Enterobacter arogene. KeywordsRaw milk, Pasteurized milk, Methylene blue reduction test, Standard plate count, Coliform Food Science and Technology Letters ISSN: 0976-982X & E-ISSN: 0976-9838, Volume 3, Issue 1, 2012 Introduction Milk is an essential part of diet for the expectant mothers as well as growing children. Milk being nutritious food for human being, also serves as a good medium for the growth of many microorganism, especially E. aerogenes specious [8], Bacterial contamination of raw milk can originated from different sources such as air, milking equipment, feed, soil, faeces and grass. The number and type of microorganism in milk immediately often after milking are affected by a different factors such as animals and equipment cleanless, season feed, animal health. It is hypothesized that differences in feeding and housing statergies of cows may influence the microbial quality of milk. Rinsing of milking machine and milking equipment with a unclear water may also be one of the reason for the presence of number of microorganism including pathogen in a raw milk [4]. The presence of these pathogenic bacteria in a milk often emerge as a as a major public health concern, especially for those individuals. Keeping a fresh milk at an a elevated temperature together with unhygienic practices in the milking process may also results in microbiological inferior quality. Coliform (Escherichia coli) are frequently used in the microbiological analysis of milk is essential to find the degree of contamination with diction and enumeration of indicator organism. The colliform bacteria are able to grow well in a variety of substrate and to utilize a number of carbohydrate and some other organic compound as a food for energy and number of fairly simple nitrogenous compound as a source of nitrogen [9]. Milk and dairy product harbour a naturally microbial flora and other microorganism, which vary within the wide range of product available on market. The origin of contamination by pathogenic bacteria, various within the product and a mode of production and processing. Contamination of milk and dairy product by pathogenic microorganisms can be an endogenous origin, following excretion from the udder of an infected an animal, through the direct contact with infected herds or through the environment [10]. Treatment and processing of milk an inhibitor encourage the multiplication of microorganism. The bacteria most frequently involved coliform. Nagpur and wardha region is suitabled in the center of India. Nagpur having a zeromile stones of India. The present study has been designed to asses, milk quality with reference to coliforms. Materials and Methods Sample Collection The 10 raw samples were collected from (Cow and Buffelo), milk vendor and 10 Pasteurized milk sample were collected from (Milk vendor, milk society shops, processing unit etc), in a sterile screw cap bottle. These sample collected from Wardha and Nagpur region. These sample were serially diluted, It spread on Eosine methylene blue media. Bismuth sulfide agar media for isolation of E.coli, Enterobacter aerogen and S.typhi. Isolation of Microorganisms from Milk Sample The milk sample were mix well before dilution. The samples were serially diluted in 1:1000 by using sterilized phasphate buffer waCitation: Aaglave P.P. and Wadatkar C.M. (2012) Microbial Examination of Milk Sample from Nagpur Region with Reference to Coliform. Food Science and Technology Letters, ISSN: 0976-982X & E-ISSN: 0976-9838, Volume 3, Issue 1, pp.-24-26. Copyright: Copyright©2012 Aaglave P.P. and Wadatkar C.M. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.